Heraeus Seminar, Jacobs 2010

	Preliminary program
Sunday, July 4th
17:00 - 17:30	Opening
17:30 - 18:30	Klaus Schulten (Theoretical and Computational Biophysics Group, Urbana, Illinois, USA) Molecular dynamics studies of unusual membrane transport
19:00 -	Barbecue

Monday, July 5th
09:00 - 09:40	Eitan Bibi (Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel) How do E. coli cells make membrane proteins?
09:40 - 10:20	Jörg H. Kleinschmidt (Universität Konstanz, Fachbereich Biologie, Konstanz, Germany) Effects of periplasmic folding factors on the insertion and folding of outer membrane proteins into lipid membranes
10:20 - 10:40	Coffee break
10:40 - 11:20	Dirk Linke (Max Planck Institute for Developmental Biology, Protein Evolution, Tübingen, Germany) Structure, domain diversity and evolution of trimeric autotransporter adhesins
11:20 - 12:00	Eric Hajjar (Biozentrum, University of Basel, SIB Swiss Institute of Bioinformatics, Basel, Switzerland) Antibiotics influx: Insights from bioinformatics, molecular modeling and atomistic simulations
12:00 - 12:30	Robert Schulz (Computational Physics and Biophysics, School of Engineering and Science, Jacobs University Bremen, Germany) Closed and open conformations of TolC: Approaches to estimate transitions
12:30 - 14:00	Lunch break
14:00 - 14:40	Klaus Fendler (Max-Planck-Institute of Biophysics, Dept. of Biophysical Chemistry, Frankfurt/Main) Working its fingers to the bone: The chloride/proton exchanger ClC-7 and bone disease
14:40 - 15:20	Christian Kandt (Computational Structural Biology, Life Science Informatics, B-IT, Bonn, Germany) Nutrient uptake & toxin export - insights from computational structural biology
15:20 - 16:00	Herbert P. Schweizer (Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, USA) Characterization of a substrate-induced multidrug efflux pump in Burkholderia pseudomallei
16:00 - 16:30	Coffee break and Poster session
16:30 - 17:10	Laure Yatime (Centre for Structural Biology, Department of Molecular Biology, University of Aarhus, Århus, Denmark) Structural insights into the high affinity binding of cardiotonic steroids to the Na+,K+-ATPase
17:10 - 17:50	Vito de Pinto (Department of Chemical Sciences, University of Catania and Instituto Nazionale di Biomembrane e Biosistemi, Catania, Italy) Beta-barrel pores, insights from the structures and lessons to design artificial pores
17:50 - 18:20	Nadine Fischer (Computational Structural Biology, Life Science Informatics, B-IT, Bonn, Germany) Mapping the proton conduction pathways in the inner membrane multi-drug translocase AcrB
18:20 - 20:00	Dinner
20:00 - 21:00	Evening lecture Roland Benz (Jacobs University Bremen, School of Engineering and Science, Biotechnology, Bremen, Germany) Clostridium botulinum C2 toxin - Identification of the binding site for chloroquine and related compounds and influence of the binding site on properties of the C2II channel

Tuesday, July 6th
09:00 - 09:40	Attilio V. Vargiu (SLACS & Department of Physics, University of Cagliari, Monserrato, Italy) Molecular modeling of antibiotic translocation coupled to functional rotation in AcrB
09:40 - 10:20	Klaas M. Pos (Institute of Biochemistry, Goethe-University Frankfurt am Main, Frankfurt am Main, Germany) The AcrB drug/H+ antiporter: The path of the proton
10:20 - 10:40	Coffee break
10:40 - 11:20	Horst Vogel (Ecole Polytechnique Fédérale de Lausanne, Institut des Sciences et Ingénierie Chimiques, Lausanne, Switzerland) Conformational diffusion from ligand binding to channel gating in the nicotinic acetylcholine receptor
11:20 - 12:00	Anna Seelig(Biozentrum der Universität Basel, Biophysikalische Chemie, Basel, Switzerland) Exploring the P-glycoprotein substrate binding cavity with polyoxyethylene alkyl ethers
12:00 - 12:30	Kozhinjampara R. Mahendran (Biophysical Chemistry, School of Engineering and Science, Jacobs University Bremen, Germany) Molecular transport across porins: Revealing fast kinetics
12:30 - 14:00 14.00 – 14.40	Lunch break Christine Ziegler (Max Planck Institute of Biophysics, Structural Biology, Frankfurt am Main, Germany) Mechanism of glycine betaine transport, osmosensing and regulation in the Na+-coupled symporter BetP
14:40 - 15:20	Ben Luisi (Department of Biochemistry, University of Cambridge, UK) Structure and recognition of the outer membrane protein component of an efflux pump
15:20 - 16:00	Frank Bernhard (Centre of Biomolecular Magnetic Resonance, Johann Wolfgang Goethe-University of Frankfurt, Frankfurt/Main) New applications of cell-free expression technologies for the production of membrane proteins
16:00 - 16:30	Coffee break and Poster session
16:30 - 17:10	Jean-Marie Pagès (Faculte de Medecine, Université de la Méditerranée, Marseille, France) Bacterial transporters: the Pandora box of antibiotic resistance
17:10 - 17:30	Birte Arntjen (Institut für Pharmazie, Hamburg, Germany) Evidence for a role of marA and marR mutations in multidrug resistance efflux and bacterial fitness
17:30 - 17:50	Franziska Pott (Institut für Pharmazie, Hamburg, Germany) Multidrug resistance efflux in Salmonella Typhimurium and Salmonella Hadar: Regulation of the AcrAB-TolC efflux pump expression by transcriptional regulators RamA and RamR
17:50 - 19:30	Dinner
19:30 – 20:30	Evening lecture Shimon Schuldiner (Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Israel) EmrE, a model for studying evolution and mechanism of ion-coupled transporters

Wednesday, July 7th
09:00 - 09:40	György Hajós (Chemical Research Center, Institute of Biomolecular Chemistry, Hungarian Academy of Sciences, Budapest, Hungary) Diverse world of heterocyclic chemical structure of MDR modulators
09:40 - 10:20	Sandrine Alibert (Faculté de Pharmacie, Université de la Méditerranée, Marseille, France) Antibiotic efflux question: the Chemistry response
11:45 - 12:45	Lunch
13:00 -	Excursion to Bremerhaven Bremerhaven 8° Ost (http://klimahaus-bremerhaven.eu/)

Thursday, July 8th
09:00 - 09:40	Leonard Amaral (Unit of Mycobacteriology, UPMM, Institute of Hygiene and Tropical Medicine of Lisbon, Universidade Nova de Lisboa, Lisboa, Portugal) Thioridazine, energetics and regulation of efflux pump genes
09:40 - 10:20	Jacques-Philippe Colletier (Laboratoire de Biophysique Moléculaire, Institut de Biologie Structurale, Grenoble, France) X-ray crystallographic structure of Pst1, a novel porin from Providencia stuartii
10:20 - 10:40	Coffee break
10:40 - 11:20	Jürgen Bohnert (Center for Infectious Diseases and Travel Medicine, Department of Medicine, Freiburg, Germany) Characterization of RND efflux pump function and inhibition using real-time efflux assays
11:20 - 12:00	Clemens Glaubitz (Institute of Biophysical Chemistry, Centre of Biomolecular Magnetic Resonance, Johann Wolfgang Goethe-University Frankfurt, Frankfurt am Main, Germany) NMR and EPR studies on the ABC multidrug efflux pump LmrA and other transport proteins
12:00 - 12:30	Osnat Tirosh (Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel) A mutation in MdfA, an E. coli multidrug transporter, leads to a new resistance activity against divalent cationic substrates
12:30 - 14:00	Lunch break
14:00 - 14:40	Simone Weyand (Membrane Protein Laboratory, Harwell Science and Innovation Campus, Chilton, Didcot, UK) Snapshots of a membrane protein at work
14:40 - 15:20	Paula Gameiro (Faculdade de Ciências da Universidade do Porto, Porto, Portugal) Insights into fluoroquinolone uptake: a biochemical and biophysical approach
15:20 - 16:00	Séamus Fanning (Centres for Food Safety & Food-borne Zoonomics, UCD Veterinary Sciences Centre, University College Dublin, Dublin, Ireland) Multiple regulatory pathways associated with high-level ciprofloxacin and multi-drug resistance in Salmonella enterica serovar Enteritidis – involvement of ramA, other global regulators and associated fitness costs
16:00 - 16:30	Coffee break and Poster session
16:30 - 17:10	Tjerk P. Straatsma (Computational Sciences and Mathematics, Pacific Northwest National Laboratory, Richland, Washington, USA) Comparative simulation study of outer membrane proteins in different membrane models
17:10 - 17:50	Stefan Schwarz (Institute of Farm Animal Genetics, Friedrich Loeffler Institute, Neustadt-Mariensee, Germany) Efflux-mediated antimicrobial resistance among livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) ST398 strains
17:50 - 18:20	Que-Tien Tran (Biophysical Chemistry, School of Engineering and Science, Jacobs University Bremen, Germany) Porins and the antibiotic resistance in Providencia stuartii
18:20 - 20:00	Dinner
20:00 - 21:00	Evening lecture Peter Henderson (Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK) The alternating access mechanism of membrane transport exemplified by the Mhp1 protein

Friday, July 9th
09:00 - 09:40	Paul Tulkens (Cellular and Molecular Pharmacology, Catholic University of Louvain, Bruxelles, Belgium) Antibiotic transporters in Streptococcus pneumoniae and Pseudomonas aeruginosa: impact on resistance and clinical significance
09:40 - 10:20	Isabelle Broutin (Laboratoire de Cristallographie et RMN Biologiques, UMR 8015 CNRS - Faculte de Pharmacie, Paris, France) Structural and functional biology of efflux pumps from Pseudomonas aeruginosa
10:20 - 10:40	Coffee break
10:40 - 11:20	Uwe Mamat (Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Division of Structural Biochemistry, Borstel, Germany) Single amino acid substitutions in either YhjD or MsbA confer viability to 3-deoxy-D-manno-oct-2-ulosonic acid-depleted Escherichia coli
11:20 - 12:00	Rajeev Misra (Arizona State University, School of Life Sciences, Life Science Tempe, Arizona, USA) Classical and atomic genetic approaches to investigate functional assembly of the TolC-AcrAB multidrug efflux system of Escherichia coli
12:00 - 12:30	Françoise van Bambeke (Cellular and Molecular Pharmacology, Catholic University of Louvain, Bruxelles, Belgium) How do antibiotics play with multidrug transporters in eucaryotic cells?
12:30 - 14:00	Lunch break
14:00 - 14:40	Liviu Movileanu (Department of Physics, Syracuse University, College of Arts and Sciences, Syracuse, USA) The stoichiometry of a bicomponent ABC transporters
14:40 - 15:20	Mark Webber (Antimicrobial Agents Research Group, Division of Immunity and Infection, University of Birmingham, Birmingham, UK) Beyond transport: global consequences of disruption of AcrAB-TolC in Salmonella enterica serovar Typhimurium
15:20 - 16:00	Thomas Gutsmann (Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Division of Biophysics, Borstel, Germany) Membrane reconstitution systems bacterial lipid membranes
16:00 - 16:30	Coffee break and Poster session
16:30 - 17:10	Stephan Nußberger (Biophysics Department, Institute of Biology, University of Stuttgart, Germany) Protein import into mitochondria: Interactions of signal peptides with TOM
17:10 - 17:50	Antje Burse (Max Planck Institute for Chemical Ecology, Department of Bioorganic Chemistry, Jena, Germany) The exploitation of plant derived glucosides by leaf beetle larvae
17:50 - 18:20	María-Cristina Villellas (Department of Microbiology, University of Zaragoza, Medical School, and CIBER Enfermedades Respiratorias, Zaragoza, Spain) Construction of efflux pumps mutants of Mycobacterium tuberculosis
18:20 - 20:00	Dinner
20:00 - 21:00	Evening lecture Robert Hancock (Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, Canada) Structure activity relationships in cationic antimicrobial and host defence peptides

Saturday, July 10th
10:00 - 12:00	Round-table discussion
12:00 - 14:00	Lunch break

End

Heraeus Seminar 2010