Ao. Univ.-Prof. Dr. Franz Bucar
Associate Professor in Pharmacognosy
Place of business:
Institute of Pharmaceutical Sciences, Department of Pharmacognosy
Tel. +43 (0)316 380 5531
Fax +43 (0)316 380 9860
(Master of Pharmacy),
Dr. rer. nat.
Univ.-Prof. (Associate Professor),
Main Research Interrests:
Recent Publications within the Scope of ATENS:
Description of Research Activity:
Our working group focuses on the isolation and identification of antimycobacterial compounds from plants in terms of:
1. Bioassay-guided isolation of antimycobacterial compounds from plants and identification of antimycobacterial active principles of plants.
2. Compounds which potentiate the activity or modify the resistance of standard antibiotics (e. g. isoniazid and tetracycline) or ethidium bromide as a pre-screening for the bioassay-guided isolation of efflux inhibiting compounds from plants.
3. Identification of plant natural products and semi-synthetic derivatives as mycobacterial efflux inhibitors using an EtBr efflux assay, which was validated in our laboratory.
working group has great expertise with phytochemical methods and an excellent
infrastructure in this respect. The most important phytochemical equipment in
our department comprises GC-MS, LC-PDA-MS-MS, HPLCs, FCPC, and various
chromatographic methods for the isolation and purification of compounds from
plants. Furthermore we have the equipment for the preparation of plant extracts
using different methods (e. g. ASE). Our microbiological methods include
determination of minimum inhibitory concentrations (microbroth dilution assay)
of our compounds prior to modulation assays or for bioassay-guided isolation of
active compounds. A modulation assay is performed as a pre-screen for the
selection of candidates with inhibitory effects on efflux pumps. Test
fractions/extracts are screened for their synergistic effects with suitable substrates in efflux assays. In-house facilities are adapted to work with fast growing strains of mycobacteria.
bioassay-guided isolation, plant extracts, GC-MS, LC-PDA-MS, FCPC, microbroth dilution assay, fast growing strains of mycobacteria, mycobacterial efflux