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Name:

Ao. Univ.-Prof. Dr. Franz Bucar

Profession:

Associate Professor in Pharmacognosy

Place of business:

Institute of Pharmaceutical Sciences, Department of Pharmacognosy

University of Graz, Universitaetsplatz 4/1, A-8010 Graz

Tel. +43 (0)316 380 5531

Fax +43 (0)316 380 9860

e-mail: franz.bucar@uni-graz.at

Degrees granted:

Mag.pharm. (Master of Pharmacy), University of Graz, 1986

Dr. rer. nat. (PhD), University of Graz, 1990

Ao. Univ.-Prof. (Associate Professor), University of Graz, 1999

Main Research Interrests:

Recent Publications within the Scope of ATENS:

Description of Research Activity:

Our working group focuses on the isolation and identification of antimycobacterial compounds from plants in terms of:

1.       Bioassay-guided isolation of antimycobacterial compounds from plants and identification of antimycobacterial active principles of plants.

2.       Compounds which potentiate the activity or modify the resistance of standard antibiotics (e. g. isoniazid and tetracycline) or ethidium bromide as a pre-screening for the bioassay-guided isolation of efflux inhibiting compounds from plants.

3.       Identification of plant natural products and semi-synthetic derivatives as mycobacterial efflux inhibitors using an EtBr efflux assay, which was validated in our laboratory.

Our working group has great expertise with phytochemical methods and an excellent infrastructure in this respect. The most important phytochemical equipment in our department comprises GC-MS, LC-PDA-MS-MS, HPLCs, FCPC, and various chromatographic methods for the isolation and purification of compounds from plants. Furthermore we have the equipment for the preparation of plant extracts using different methods (e. g. ASE). Our microbiological methods include determination of minimum inhibitory concentrations (microbroth dilution assay) of our compounds prior to modulation assays or for bioassay-guided isolation of active compounds. A modulation assay is performed as a pre-screen for the selection of candidates with inhibitory effects on efflux pumps. Test compounds/
fractions/extracts are screened for their synergistic effects with suitable substrates in efflux assays. In-house facilities are adapted to work with fast growing strains of mycobacteria.

Keywords:

bioassay-guided isolation, plant extracts, GC-MS, LC-PDA-MS, FCPC, microbroth dilution assay, fast growing strains of mycobacteria, mycobacterial efflux

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